Protection and delivery of multiple therapeutic proteins
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsEric M. Jeffries, Noah R. Johnson, Daniel Long, Yadong Wang
Life of this patent
Estimating the term…
Abstract
Compositions are provided herein comprising a coacervate of a polycationic polymer, a polyanionic polymer, and platelet-rich plasma and/or serum, or a fraction or concentrate thereof. The composition is useful in wound healing. Compositions also are provided that comprise a hydrogel comprising TIMP-3; and a complex of a polycationic polymer, a polyanionic polymer, FGF-2 and SDF-1α embedded in the hydrogel, which is useful in treating a myocardial infarction.
Claims20
A composition comprising a coacervate of:
- a polycationic polymer,
- a polyanionic polymer, and
- a fraction or concentrate of platelet-rich plasma wherein platelets of platelet-rich plasma are activated to produce a fibrin clot, and wherein:
- the fraction or concentrate of platelet-rich plasma comprises pure platelet-rich fibrin;
- the fraction or concentrate of platelet-rich plasma comprises leukocyte-rich platelet-rich fibrin;
- or
- the fibrin clot is removed and the protein content of the platelet-rich plasma is concentrated.
A method of preparing a therapeutic composition for use in treating a wound in a patient, comprising:
- a. mixing a polyanionic polymer with a fraction or concentrate of platelet-rich plasma, wherein platelets of platelet-rich plasma are activated to produce a fibrin clot, and wherein:
- the fraction or concentrate of platelet-rich plasma comprises pure platelet-rich fibrin;
- the fraction or concentrate of platelet-rich plasma comprises leukocyte-rich platelet-rich fibrin;
- or
- the fibrin clot is removed and the protein content of the platelet-rich plasma is concentrated;
- and
- b. mixing a polycationic polymer with the mixture of the polyanionic polymer and the fraction or concentrate of platelet-rich plasma.
Dependencies are inferred from the claim text.