Isolates from spent turmeric for the management of rheumatoid arthritis

A method of treating rheumatoid arthritis in mammals, said method comprising step of administering to said mammals effective amounts of spent turmeric protein or spent turmeric starch, said protein and starch obtained by process comprising steps of

• a) Extracting spent turmeric with water wherein ratio of spent turmeric and water is from about 1:10 to about 1:50, at a temperature 65° C. for 6 hours with occasional stirring to get water extract of spent turmeric and a residual material, wherein the residual material is spent turmeric material-2;
• b) Centrifuging the water extract of step (a) to trap sand or dust particles to obtain a clear filtrate;
• c) Concentrating the clear filtrate of step (b) at 60° C. with high vacuum to obtain a concentrate and further cooling said concentrate;
• d) Adding acetone, to the concentrate of step (c) wherein ratio of acetone and concentrate, is 1:2, followed by occasional stirring, incubation at room temperature for 4 hours to form acetone precipitated protein and allowing the precipitated protein to completely settle down with a supernatant above;
• e) Decanting the supernatant of step (d) without disturbing the precipitated protein followed by filtration, washing the precipitated protein with acetone to remove moisture and filtering again;
• f) Drying protein precipitate of step (e) immediately at 60° C. with high vacuum to obtain fine protein (glycoprotein) powder as an extract, wherein the extract reduces inflammation and symptoms of rheumatoid arthritis in said mammals including pain, swelling, oedema and reduction in serum markers of IL-6 and TNF-α;
• g) Subjecting the residual spent turmeric material-2 of step (a) devoid of protein of step (f) to washing with soaking volume of Acetone and Methanol for 4 hours each at 46° C. and 30° C. respectively to remove traces of curcuminoids and oxalic acid content to form washed spent turmeric material-2;
• and
• h) Drying washed spent turmeric material-2 of step (g) for 24 hours to remove residue solvents followed by powdering and sieving to get turmeric starch (polysaccharide) as an extract wherein polysaccharide is attached to hydrophobic protein, wherein the extract reduces inflammation and symptoms of rheumatoid arthritis in said mammals including pain, swelling, oedema and reduction in serum markers of IL-6 and TNF-α.