Total flavonoids extract of Gynura formosana Kitam., preparation method therefor and use thereof for treating hyperuricemia

A method for treating hyperuricemia or gout, comprising the step of administering to a subject in need a pharmaceutical preparation comprising a total flavonoid extract from Gynura formosana Kitam. as an active ingredient, wherein the total flavonoid extract from Gynura formosana Kitam., comprises, in weight percent, 80-85% of rutin,

• wherein the active ingredient is mixed with a conventional auxiliary material and prepared according to a conventional process into clinically acceptable forms selected from the group consisting of tablets, capsules, powders, mixtures, pills, granules, syrups, plasters, suppositories, aerosols, ointments and injections;
• and
• wherein the total flavonoid extract from Gynura formosana Kitam. is prepared by the steps of:
• (1) Extraction:
• extracting Gynura formosana Kitam. with an extraction solvent to obtain an extraction solution, and adjusting the extraction solution to a pH of 4-8 to obtain a reaction solution:
• (2) Enzymolysis:
• adding a complex enzyme into the reaction solution to carry out enzymolysis through a forced circular reaction at a temperature of 30° C. to 50° C. for 1 to 4 hours, then carrying out suction filtration, and collecting a filtrate;
• (3) Extraction and Concentration:
• extracting the filtrate by using a macroporous resin A to obtain an extracted solution, and concentrating the extracted solution to obtain a concentrated solution;
• and
• (4) Separation and Purification:
• centrifuging the concentrated solution, collecting a supernatant and carrying out elution by using a macroporous resin B, measuring absorbance at a wavelength of 510 nm, collecting eluate, concentrating and drying the eluate to obtain an extract.