US 11,298,385 B2 · Microorganism for delivering drug for treatment of gastrointestinal disease, which expresses and secretes P8 protein, and pharmaceutical composition for preventing or treating gastrointestinal disease, which includes the same

Abstract

The present invention relates to a microorganism for drug delivery, which has been transformed with a gene construct comprising a therapeutically active peptide, which can be delivered safely into the intestines through oral administration, and which can express and secrete the therapeutic peptide in the gastrointestinal tract, and also relates to a pharmaceutical composition for prevention or treatment of gastrointestinal disease, which includes the same. The present invention is directed to a lactic acid bacteria drug delivery system capable of overexpressing and secreting a lactic acid bacteria-derived anticancer protein, which is developed by introducing the anticancer protein into a lactic acid bacteria expression and secretion system. It is expected that the lactic acid bacteria drug delivery system will be widely used as a natural protein therapeutic agent against gastrointestinal disease in the medical field.

Claims9

Dependent claims

A Pediococcus pentosaceus strain for treating colorectal cancer, comprising a DNA construct that comprises:

an exogenous promoter;
a polynucleotide encoding a P8 protein operably linked to the exogenous promoter and having the nucleotide sequence represented by SEQ ID NO:
1;
and
a gene encoding a secretion signal peptide,
wherein the Pediococcus pentosaceus strain is capable of expressing and secreting the P8 protein in a gastrointestinal tract, and
the exogenous promoter is at least one promoter selected from the group consisting of SEQ ID NO:
3 to SEQ ID NO:
8.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/471,940Filed 16 Oct 2018

Priority

KR 10-2018-00030089 Jan 2018

Published

US 2019/0328801 A131 Oct 2019

Granted

12 Apr 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification20 codes

A61K 35/747
Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs: lactobacilli, e.g. L. acidophilus or L. brevis
A61K 35/744
Probiotics: lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
A61K 35/745
Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs: bifidobacteria
A61K 38/164
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria

Microorganism for delivering drug for treatment of gastrointestinal disease, which expresses and secretes P8 protein, and pharmaceutical composition for preventing or treating gastrointestinal disease, which includes the same

Life of this patent

Microorganism for delivering drug for treatment of gastrointestinal disease, which expresses and secretes P8 protein, and pharmaceutical composition for preventing or treating gastrointestinal disease, which includes the same

Life of this patent

Abstract

Claims9