Cell penetrating peptide (CPP)-mediated EV loading
AssigneeEvox Therapeutics Ltd
InventorsOscar Wiklander, Per Lundin, Dhanu Gupta
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Abstract
The present invention relates to methods for loading extracellular vesicles (EVs) with a pharmacological agent. The invention discloses the use of cell-penetrating peptides as carriers into EVs, using either a non-covalent or covalent loading approach. Furthermore, the present invention pertains to medical uses and compositions comprising such pharmacological agent-loaded EVs.
Claims6
A method for loading exosomes with at least one pharmacological agent, comprising exposing a population of exosomes to at least one pharmacological agent and at least one cell-penetrating peptide (CPP), wherein the at least one pharmacological agent and the at least one CPP are present in the form of a non-covalent complex.
A method for loading an exosome with at least one pharmacological agent, comprising the steps of:
- i. exposing a population of exosome source cells to the at least one pharmacological agent and at least one cell-penetrating peptide (CPP), wherein the at least one pharmacological agent and the at least one CPP are present in the form of a non-covalent complex;
- and,
- ii. harvesting exosomes produced by the exosome source cells, wherein the exosomes comprise said pharmacological agent.
Dependencies are inferred from the claim text.