One-step method for production of ultra-small lipid structures
AssigneeFountain Technologies International, LLC
InventorMichael Fountain
Life of this patent
Estimating the term…
Abstract
Ultra-small Lipid Structures (USLS) with an average mean particle diameter of less than 100 nm are made using a single step process by diluting a hydro-organic solution containing lipids and passenger compounds. These particles are capable of sequestering the passenger molecules and self-assemble in a single process step into USLS. The USLS have applications in, for example, agricultural, cosmetics, pharmaceutical and food and beverage industries.
Claims30
A method for preparing at least one ultra-small lipid structure (USLS) comprising the step of diluting a solution, in a single dilution process step, with water at a dilution ratio of about 1:5 to about 1:200,
- wherein the solution comprises:
- (a) a hydro-organic solvent mixture containing about 0.1% to about 20% (v/v) water and about 80% to about 99.9% (v/v) of one or more water-miscible organic solvents;
- (b) about 50 mg/ml to about 250 mg/ml of one or more lipid compounds, wherein prior to diluting the solution, none of the one or more lipid compounds in the solution have a closed lipid structure;
- and
- (c) one or more passenger compounds, wherein the at least one ultra-small lipid structure has a closed lipid structure comprising at least one of the one or more passenger compounds sequestered therein as formed during the single dilution process step, and
- wherein the term “ultra-small lipid structures (USLS)” refers to lipid vesicles having a bilayer or non-bilayer lipid surrounding structure and an aqueous or semi-aqueous core, and having an average diameter of 100 nm or less as measured using a dynamic light scattering (DLS) analyzer,
- wherein the USLS product obtained contains a population of from 1% to 2% particles having diameter of from 1 nm to 5 nm as measured using a dynamic light scattering (DLS) analyzer.
A method for preparing at least one ultra-small lipid structure (USLS) comprising the step of diluting a solution, during a single step dilution process, with water at a dilution ratio of about 1:5 to about 1:200,
- wherein the solution comprises:
- (a) a hydro-organic solvent mixture containing about 0.1% to about 20% (v/v) water and about 80% to about 99.9% (v/v) of one or more water-miscible organic solvents;
- (b) about 50 mg/ml to about 250 mg/ml of one or more lipid compounds, wherein prior to diluting the solution, none of the one or more lipid compounds in the solution have a closed lipid structure;
- and
- (c) one or more passenger compounds,
- wherein the at least one ultra-small lipid structure has a closed lipid structure comprising at least one of the one or more passenger compounds internally sequestered therein as formed during the single step dilution process, and
- wherein the term “ultra-small lipid structures (USLS)” refers to lipid vesicles having a bilayer or non-bilayer lipid surrounding structure and an aqueous or semi-aqueous core, and having an average diameter of 100 nm or less as measured using a dynamic light scattering (DLS) analyzer.
Dependencies are inferred from the claim text.