Surface tension mediated lyo-processing technique for preservation of biologics
AssigneeThe Regents of the University of Michigan
InventorNilay Chakraborty
Life of this patent
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Abstract
Methods and devices for lyo-processing biological materials are provided. The methods include submerging the biological molecules or cells in a buffer solution comprising trehalose, withdrawing the biological molecules or cells from the buffer solution in an environment that does not include oxygen to generate lyo-stabilized biological molecules or cells, and storing the lyo-stabilized biological molecules or cells. The devices include a motor that lowers a horizontal member having a clamp for receiving a substrate toward a solvent reservoir and raises the horizontal member having the clamp up and away from the solvent reservoir.
Claims19
A method for lyo-processing a biological material, the method comprising:
- generating a layer of the biological material on a substrate;
- submerging the substrate in a lyo-processing solution comprising a lyoprotectant;
- raising the substrate out of the lyo-processing solution;
- and
- contacting the biological material with an inert gas as the substrate is raised out of the lyo-processing solution to generate a lyo-stabilized biological material on the substrate.
A method for lyo-processing a biological material, the method comprising:
- submerging the biological material comprising biological molecules or cells disposed on a substrate in a lyo-processing solution comprising trehalose, betaine, and choline, the lyo-processing solution being substantially free of Na+;
- raising the biological molecules or cells disposed on the substrate out of the lyo-processing solution;
- and
- passing the biological material disposed on the substrate through an environment that is substantially free of oxygen to lyo-stabilize the biological material.
A method for lyo-processing a biological material, the method comprising:
- generating a layer of the biological material on a substrate;
- submerging the substrate in a lyo-processing solution comprising a lyoprotectant;
- withdrawing the substrate from the lyo-processing solution at a rate of greater than or equal to about 5 mm/min to less than or equal to about 500 mm/min;
- and
- contacting the biological material with an inert gas as the substrate is withdrawn from the lyo-processing solution to generate a lyo-stabilized biological material on the substrate.
Dependencies are inferred from the claim text.