Nucleic acid control molecules from non-human organisms
AssigneeExact Sciences Development Company, LLC
InventorsHatim T. Allawi, Graham P. Lidgard, Brian Aizenstein, Melissa M. Gray
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Abstract
The present invention provides synthetic DNA strands that find use as process controls in DNA processing and nucleic acid testing methods. In particular, provided herein are synthetic methylated DNA strands of known composition for use as control molecules in DNA testing, e.g., of mutations and/or methylation of DNA isolated from non-fish samples, such as human samples.
Claims16
A composition comprising a) isolated methylated zebrafish DNA or synthetic methylated DNA comprising a zebrafish DNA nucleotide sequence, and b) a second component, in a mixture, wherein said second component is selected from:
- methylated DNA from a biological sample from a human;
- a eukaryotic cell from a human;
- and
- a biological sample from a human.
A method of treating a biological sample containing methylated DNA from a human subject, comprising
- a) combining isolated methylated zebrafish DNA or synthetic methylated DNA comprising a zebrafish DNA nucleotide sequence with said biological sample in a mixture;
- and
- b) treating said mixture to purify DNA from said mixture.
Dependencies are inferred from the claim text.