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US 11,149,322 B2Granted
Methods and compositions for human papillomaviruses and sexually transmitted infections detection, identification and quantification
AssigneeChapter Diagnostics, Inc.
InventorsChunlin Wang, Zhihai Ma, Baback Gharizadeh
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Abstract
The present disclosure relates to compositions and methods for detection, identification and quantification of human papillomaviruses (HPV) and clinically significant sexually transmitted infections (STI) in a single amplification reaction. The disclosed method utilizes next-generation sequencing (NGS) to sequence amplified products. The present disclosure is also directed to kits containing primers specific to multiple HPV and STI targets.
Claims5
A method of screening a sample from each of one or more subjects, comprising the steps of:
- for each sample, hybridizing a plurality of target-specific primers with nucleic acid from the sample in the presence of barcoded universal primers to form a test reaction, wherein at least one target-specific primer is configured to bind to a target sequence specific to sexually transmitted infection and wherein each target-specific primer comprises a specific sequence portion directed to a target nucleic acid sequence and a universal priming portion;
- subjecting each test reaction to amplification conditions to generate amplicons;
- subjecting at least a portion of the amplicons generated from each sample to bead cleanup to form enriched amplicons;
- and
- sequencing the enriched amplicons, formed from each sample, by next-generation sequencing;
- wherein each barcoded universal primer comprises:
- a universal priming portion at the 3′-end;
- a barcode portion in the middle;
- and a universal priming portion at the 5′-end.
6 more elements
Dependencies are inferred from the claim text.