Enzyme immobilized adhesive layer for analyte sensors
AssigneeDexCom, Inc.
InventorsShanger Wang, Ted Tang Lee, Jiong Zou
Life of this patent
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Abstract
Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.
Claims28
A device for measurement of an analyte concentration, the device comprising:
- a sensor configured to generate a signal associated with a concentration of an analyte;
- a sensing membrane located over the sensor, the sensing membrane comprising an enzyme layer, wherein the enzyme layer comprises an enzyme and an enzyme-layer polymer that retains at least 80% of initial enzyme loading when contacted with an aqueous solution for 1 hour at about 25° C., and wherein the enzyme-layer polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units, and wherein at least one of the zwitterionic repeating units is an internal zwitterionic repeating unit in a backbone of the enzyme-layer polymer and the at least one of the zwitterionic repeating units compromises a betaine compound or a precursor thereof;
- and
- a biointerface membrane positioned over the sensing membrane and configured to interface with a biological fluid.
A device for measurement of an analyte concentration, the device comprising:
- a sensor configured to generate a signal associated with a concentration of an analyte;
- a sensing membrane located over the sensor, the sensing membrane comprising an enzyme layer, wherein the enzyme layer comprises an enzyme and an enzyme-layer polymer that retains at least 50% of initial sensor sensitivity when exposed to 70° C. and 95% relative humidity conditions for up to 24 hours treatment, and the enzyme-layer polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units wherein at least one zwitterionic repeating unit is an internal zwitterionic repeating unit in a backbone of the enzyme-layer polymer, and the at least one zwitterionic repeating unit comprises betaine compound or a precursor thereof;
- and
- a biointerface membrane positioned over the sensing membrane and configured to interface with a biological fluid.
Dependencies are inferred from the claim text.