Method for observing an object

A device for observing an object, present in a sample, comprising:

• a support, configured to receive the sample;
• a light source, configured to emit an incident light beam, in order to illuminate the object;
• an image sensor, configured to acquire an image representative of radiation back scattered by the object under the illumination by the incident light beam;
• wherein the device further comprises:
• a screen, facing the support, so as to be exposed to radiation back scattered by the object when the object is illuminated by the incident light beam, so as to form, on the screen, a scattergram, representative of said back-scattered radiation;
• the screen comprising a first face exposed to the back-scattered radiation;
• the screen disposed between the light source and the object, and comprising an aperture, through which the incident light beam propagates before reaching the object, the screen comprising a second face, so that the scattergram formed on the first face appears on the second face;
• the image sensor being configured to acquire an image of the scattergram formed on the screen, the screen being placed between the image sensor and the object, the image sensor being coupled to the second face of the screen by a focusing optic;
• wherein
• the device further comprises a reflective element, configured to direct the incident light beam emitted by the light source toward the object, through the aperture, the reflective element being placed between the object and the image sensor,
• the image sensor, the reflective element, the aperture, and the object are aligned according to a single axis of incidence, along which the incident light beam propagates toward the object,
• (i) the reflective element absorbs at least 30% of the back-scattered radiation emitted by the object, along the axis of incidence, or (ii) the reflective element is fastened to a holder, the holder absorbing at least 30% of the back-scattered radiation emitted by the object, along the axis of incidence, and
• the image of the scattergram comprises a shadow, formed by the reflective element and/or the holder, the shadow blocking a specular reflection of the incident light beam.