Methods of detecting autoimmune or immune-related diseases or conditions
AssigneePresident and Fellows of Harvard College
InventorAmin I. Kassis
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Abstract
This invention provides methods of using phagocytic cells alone or in combination with non-phagocytic cells in the diagnosis, prognosis, or monitoring of autoimmune or immune-related diseases or conditions. The invention also provides methods of using phagocytic cells alone or in combination with non-phagocytic cells to identify markers of autoimmune or immune-related diseases or conditions.
Claims5
An assay for detecting a marker of an autoimmune or immune-related disease or condition in a human subject, wherein the autoimmune or immune-related disease or condition is multiple sclerosis or inflammatory bowel disease, the assay comprising:
- a) obtaining a macrophage having a DNA content of more than 2n (>2n macrophage cell) from the blood of the human subject, wherein the >2n macrophage has been separated from phagocytic cells having a DNA content of 2n (=2n phagocytic cells), and detecting a first gene expression profile of the marker of an autoimmune or immune-related disease or condition;
- and
- b) obtaining a non-phagocytic cell from the blood of the human subject and detecting a second gene expression profile of the marker of an autoimmune or immune-related disease or condition in the obtained non-phagocytic cell.
Dependencies are inferred from the claim text.