US 11,111,536 B2 · Method for determining cross contamination of mouse genes for human cells in a patient-derived xenograft cells

Abstract

The present application relates to a detection kit for genotypes capable of confirming cross contamination that may occur in a banking process of a patient-derived xenograft model or cell-derived xenograft model and a method for determining cross contamination using the same. According to the present disclosure, it is possible to determine all of cross contamination of mouse related genes, have high detection sensitivity and specificity to be close to 100%, rapidly examine the contamination, and be very useful in predicting mouse contamination. Therefore, according to the present disclosure, cross contamination of genes related with the human and the mouse is predicted in advance to be applied to evaluation of anticancer drug efficacy using a patient-derived xenograft model or cell-derived xenograft model and contribute to cell banks using the patient-derived xenograft model or cell-derived xenograft model, and as a result, the present disclosure is very useful in a medical industry.

Claims4

Dependent claims

A method for determining cross contamination of mouse genes for human cells in a method for determining patient-derived xenograft cells, the method comprising the steps of:

amplifying a human papola gene and a mouse papola gene using oligonucleotide primers consisting of base sequences of SEQ ID NOS:
5 to 8 by a PCR method;
hydrolyzing oligonucleotide probes consisting of base sequences of SEQ ID NOS:
11 and 12;
and
detecting a labeling means binding to the probes and determining a ratio of the human papola gene and the mouse papola gene to determine cross contamination.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/666,449Filed 29 Oct 2019 · division

Priority

KR 10-2016-00544583 May 2016

Published

US 2020/0102614 A12 Apr 2020

Granted

7 Sep 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6881
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for tissue or cell typing, e.g. human leukocyte antigen [HLA]…
C12Q 1/6888
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for detection or identification of organisms
C12Q 2600/158
Oligonucleotides characterized by their use: expression markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Method for determining cross contamination of mouse genes for human cells in a patient-derived xenograft cells

Life of this patent

Method for determining cross contamination of mouse genes for human cells in a patient-derived xenograft cells

Life of this patent

Abstract

Claims4