Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
AssigneeQiagen GmbH
InventorsNiels B. Ramsing, Peter Mouritzen, Søren Morgenthaler Echwald, Niels Tolstrup
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Abstract
The invention relates to nucleic acid probes, nucleic acid probe libraries, and kits for detecting, classifying, or quantitating components in a complex mixture of nucleic acids, such as a transcriptome, and methods of using the same. The invention also relates to methods of identifying nucleic acid probes useful in the probe libraries and to methods of identifying a means for detection of a given nucleic acid.
Claims17
A library of oligonucleotide probes, wherein each probe in the library comprises a recognition sequence tag and a detection moiety, wherein the recognition sequence tag has at least one modified monomer analogue that increases the binding affinity for the complementary target sequence relative to the corresponding unmodified oligodeoxyribonucleotide,
- wherein each probe has sufficient stability for sequence-specific binding and detection of its target sequence under stringent conditions,
- wherein each probe has independently a total of 8 or 9 nucleotides,
- wherein the library comprises a total of from 50 to 500 probes,
- wherein the probes in the library hybridize to more than 90% of the mRNAs set forth in SEQ ID NOS:
- 47-35734, and
- wherein at least one modified monomer analogue is a non-naturally occurring nucleotide analogue, a deoxyribose or ribose analogue, or an internucleotide linkage other than a phosphodiester linkage.
Dependencies are inferred from the claim text.