US 11,111,528 B2 · Method and apparatus for ultrasensitive quantification of microRNA using an atomic force microscope and a hybrid binding domain

Abstract

The present invention provides an apparatus and a method for detecting the presence of and/or determining the amount of a label-free microRNA using an atomic force microscope. The method is extremely selective and/or ultrasensitive. In particular, the present invention provides a cantilever comprising a probe that selectively binds to a double strand of DNA/RNA hybrid complex. The probe comprises a hybrid binding domain (HBD) or a variant thereof.

Claims17

Dependent claims

An atomic force microscope comprising a cantilever, wherein said cantilever comprises a probing tip and a probe that is immobilized on said probing tip, wherein said probe comprises a hybrid binding domain (HBD) or a variant thereof, wherein said variant of HBD is elected from the group consisting of:

an HBD linked to a glutathione S-transferase (GST);
an HDB linked to a histidine-tag;
and
a biotinylated HBD,
and wherein said HBD is capable of binding to a minor groove of a DNA/RNA hybrid duplex.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/412,025Filed 22 Jan 2017

Priority

KR 10-2015-016410023 Nov 2015

Published

US 2017/0233799 A117 Aug 2017

Granted

7 Sep 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6816
Hybridisation assays: characterised by the detection means
C12Q 1/6837
Enzymatic or biochemical coupling of nucleic acids to a solid phase: using probe arrays or probe chips
G01Q 60/38
AFM [Atomic Force Microscopy] or apparatus therefor, e.g. AFM probes: probes, their manufacture, or their related instrumentation, e.g. holders
G01Q 60/42
Probes, their manufacture, or their related instrumentation, e.g. holders: functionalisation

Method and apparatus for ultrasensitive quantification of microRNA using an atomic force microscope and a hybrid binding domain

Life of this patent

Method and apparatus for ultrasensitive quantification of microRNA using an atomic force microscope and a hybrid binding domain

Life of this patent

Abstract

Claims17