US 11,111,520 B2 · Compositions and methods for enriching populations of nucleic acids

Abstract

This disclosure provides methods and compositions that are useful for enriching a particular population of nucleic acids (a “population of interest”) within a complex mixture of nucleic acids. The population of interest may make up a minor portion of a complex mixture of nucleic acids. The methods and compositions provided herein are useful for detecting, predicting, diagnosing, or monitoring a disease or disorder, particularly a disease or disorder caused by a foreign microbe or pathogen.

Claims49

Dependent claims

A method of enriching microbial cell-free nucleic acids, the method comprising:

(a) identifying nucleic acid length ranges at which a ratio of microbial cell-free nucleic acid to host cell-free nucleic acid is favored;
(b) providing a host sample wherein the sample is selected from the group consisting of blood, plasma, serum, saliva, cerebrospinal fluid, synovial fluid, and lavage wherein the host sample comprises host cell-free nucleic acids and the microbial cell-free nucleic acids;
and
(c) enriching the microbial cell-free nucleic acids relative to the host cell-free nucleic acids by enriching for nucleic acid length ranges at which a ratio of microbial cell-free nucleic acid to host cell-free nucleic acid is favored.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/157,374Filed 17 May 2016 · us provisional application

Priority

18 May 2015

Published

US 2017/0016048 A119 Jan 2017

Granted

7 Sep 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6888
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for detection or identification of organisms
C12Q 1/6804
Involving nucleic acids: nucleic acid analysis using immunogens
C12Q 1/6809
Involving nucleic acids: methods for determination or identification of nucleic acids involving differential detection

Compositions and methods for enriching populations of nucleic acids

Life of this patent

Compositions and methods for enriching populations of nucleic acids

Life of this patent

Abstract

Claims49