Method for expressing protein gene in response to expression of miRNA
AssigneeKyoto University
InventorsHirohide Saito, Yoshihiko Fujita
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Abstract
An mRNA forcibly expresses a protein gene in response to a miRNA, and a method for forcibly expressing the same, are provided. An artificial mRNA comprising a sequence encoding a protein gene, a miRNA target sequence linked to the 3′-terminal side of a Poly A sequence, and a translational repression sequence linked to the 3′-terminal side of the miRNA target sequence; and a method for expressing a protein gene in response to the expression of a miRNA, comprising a step of introducing the artificial mRNA into a cell.
Claims19
An artificial mRNA comprising:
- a sequence encoding a protein,
- a miRNA target sequence linked to the 3′-terminal side of a Poly A sequence of at least 50 nucleotides, and
- a translational repression sequence linked to the 3′-terminal side of the miRNA target sequence.
A method for determining a desired cell type from a cell group comprising two or more cells, using the expression of a miRNA as an indicator,
- the method comprising the following steps:
- (1) a step of introducing a first artificial mRNA comprising a sequence encoding a first marker, a first miRNA target sequence linked to the 3′-terminal side of a Poly A sequence of at least 50 nucleotides, and a translational repression sequence linked to the 3′-terminal side of the first miRNA target sequence, into the cell group;
- and
- (2) a step of determining the cell type, using the translation level of the first marker as an indicator.
Dependencies are inferred from the claim text.