ToLCNDV resistant melon plants

A method for producing hybrid cultivated melon seeds comprising

• a) crossing a first ToLCNDV resistant inbred melon plant comprising two chromosomes 11 each having an introgression fragment on chromosome 11 of a ToLCNDV resistant donor plant, the introgression fragment comprising QTL11 and a Thymine for SNP_03 at nucleotide 101 of SEQ ID NO:
• 3, or the sequence of the donor plant in-between SNP_03 at nucleotide 101 of SEQ ID NO:
• 3 and SNP_04 at nucleotide 101 of SEQ ID NO:
• 4, or in-between SNP_02 at nucleotide 101 of SEQ ID NO:
• 2 and SNP_03 at nucleotide 101 of SEQ ID NO:
• 3;
• or the introgression fragment comprising QTL11 and the resistant donor genotype for one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04, wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO:
• 1, a Guanine at nucleotide 101 of SEQ ID NO:
• 2, a Thymine at nucleotide 101 of SEQ ID NO:
• 3 and a Thymine at nucleotide 101 of SEQ ID NO:
• 4, respectively
• with a second ToLCNDV resistant inbred melon plant comprising two chromosomes 11 each having an introgression fragment on chromosome 11 of a ToLCNDV resistant donor plant, the introgression fragment comprising QTL11 and a Thymine for SNP_03 at nucleotide 101 of SEQ ID NO:3, or the sequence of the donor plant in-between SNP_03 at nucleotide 101 of SEQ ID NO:3 and SNP_04 at nucleotide 101 of SEQ ID NO:
• 4, or in-between SNP_02 at nucleotide 101 of SEQ ID NO:
• 2 and SNP_03 at nucleotide 101 of SEQ ID NO:
• 3;
• or the introgression fragment comprising QTL11 and the resistant donor genotype for one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04 wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO:
• 1, a Guanine at nucleotide 101 of SEQ ID NO:
• 2, a Thymine at nucleotide 101 of SEQ ID NO:
• 3 and a Thymine at nucleotide 101 of SEQ ID NO:
• 4, respectively;
• and
• b) collecting seeds obtained from the cross of a).

A method of screening plants or plant parts, or DNA derived therefrom, for the presence of a fragment on chromosome 11 and/or 12 conferring ToLCNDV resistance, comprising:

• i) screening the genomic DNA for the SNP genotype of one or more or all of SNP_01 at nucleotide 101 of SEQ ID NO:
• 1, SNP_02 at nucleotide 101 of SEQ ID NO:
• 2, SNP_03 at nucleotide 101 of SEQ ID NO:
• 3 and SNP_04 at nucleotide 101 of SEQ ID NO:
• 4 and/or for one or more of SNP_05 at nucleotide 101 of SEQ ID NO:
• 5, SNP_06 at nucleotide 101 of SEQ ID NO:
• 6 and SNP_07 at nucleotide 101 of SEQ ID NO:
• 7;
• and
• ii) optionally selecting a plant or plant part which comprise the resistant donor genotype of one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04 and/or for one or more or all of SNP_05, SNP_06 and SNP_07, wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO:
• 1, a Guanine at nucleotide 101 of SEQ ID NO:
• 2, a Thymine at nucleotide 101 of SEQ ID NO:
• 3 and a Thymine at nucleotide 101 of SEQ ID NO:
• 4 and the resistant donor genotype for SNP_05, SNP_06 and SNP_07 is a Cytosine at nucleotide 101 of SEQ ID NO:
• 5, a Guanine at nucleotide 101 of SEQ ID NO:
• 6 and a Thymine at nucleotide 101 of SEQ ID NO:
• 7.