Mutated protoporphyrinogen IX oxidase (PPX) genes
AssigneesCibus US LLC, Cibus Europe B.V.
InventorsGregory F. W. Gocal, Peter R. Beetham, Aura Estela Gonzalez Schopke, Sarah Dumm, James Pearce, Christian Schopke, Keith A. Walker
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Abstract
Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant.
Claims8
A non-transgenic plant cell, resistant to one or more PPX-inhibiting herbicides, comprising a mutated protoporphyrinogen IX oxidase (PPX) gene, wherein said gene encodes a protein comprising a mutation at an amino acid position corresponding to position 426 of SEQ ID NO:
- 1, wherein the mutation is a Tyr to Phe substitution.
A non-transgenic plant, resistant to one or more PPX-inhibiting herbicides, comprising a mutated protoporphyrinogen IX oxidase (PPX) gene, wherein said gene encodes a protein comprising a mutation at an amino acid position corresponding to position 426 of SEQ ID NO:
- 1, wherein the mutation is a Tyr to Phe substitution.
A method for producing a non-transgenic plant cell with a mutated PPX gene, comprising introducing into a plant cell a gene repair oligonucleobase (GRON) encoding a mismatch with a protoporphyrinogen IX oxidase (PPX) gene of the plant cell under conditions that introduce a targeted mutation in the PPX gene resulting from the mismatch, to thereby produce a plant cell that expresses a PPX protein from the PPX gene comprising a mutation at an amino acid position corresponding to position of SEQ ID NO:
- 1, wherein the mutation is a Tyr to Phe substitution.
Dependencies are inferred from the claim text.