Expression system for Psicose epimerase and production for Psicose using the same

A method of producing a psicose epimerase, comprising:

• culturing a recombinant Corynebacterium sp. cell transformed by a vector comprising a gene expression cassette, and
• reacting a fructose-containing substrate with at least one selected from the group consisting of an psicose epimerase obtained from the culture of the recombinant Corynebacterium sp., a recombinant cell, a culture of the recombinant cell, a lysate of the recombinant cell and an extract of cell culture or the cell lysate, and
• wherein the gene expression cassette, producing a psicose epimerase in Corynebacterium sp., and comprising:
• a nucleotide sequence encoding the psicose epimerase;
• and
• a regulating sequence being operably connected to the nucleotide sequence in the upstream regulating the expression of the nucleotide sequence in Corynebacterium sp., and comprising a promoter, a ribosome binding site (RBS) sequence and a first spacer sequence in the direction of 5′ to 3′,
• wherein the promoter includes the nucleotide sequence of SEQ ID NO:
• 1,
• the ribosome binding site (RBS) sequence is a nucleotide sequence in a size of 7 to 20 bases including the nucleotide sequence of SEQ ID NO:
• 2, and
• the first spacer sequence is selected from the group consisting of the nucleotide sequences of SEQ ID NO:
• 4 to SEQ ID NO:
• 6.