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US 11,111,483 B2Granted
Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems and methods
AssigneeB-Mogen Biotechnologies, Inc.
InventorsDavid Largaespada, Branden Moriarity, Beau Webber, Neil Otto, Sandeep Kumar, Leah Hogdal
Life of this patent
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Abstract
This disclosure provides various TcBuster transposases and transposons, systems, and methods of use.
Claims24
A fusion transposase comprising a TcBuster transposase amino acid sequence and a DNA sequence specific binding domain, wherein the TcBuster transposase amino acid sequence has at least 70% identity to full-length SEQ ID NO:
- 1 and an amino acid substitution of D189A, V377T, E469K, K573E, E578L, or any combination thereof, when numbered in accordance with SEQ ID NO:
- 1.
A system for genome editing comprising:
- a transposon and a fusion transposase or a polynucleotide coding for the fusion transposase, wherein the fusion transposase comprises a TcBuster transposase amino acid sequence and a DNA sequence specific binding domain, wherein the TcBuster transposase amino acid sequence (i) recognizes the transposon, (ii) has at least 70% identity to full-length SEQ ID NO:
- 1, and (iii) comprises an amino acid substitution of D189A, V377T, E469K, K573E, E578L, or any combination thereof, when numbered in accordance with SEQ ID NO:
- 1.
A method of genome editing comprising:
- introducing into a cell a transposon and a fusion transposase or a polynucleotide encoding the fusion transposase, wherein the fusion transposase comprises a TcBuster transposase amino acid sequence and a DNA sequence specific binding domain, wherein the TcBuster transposase amino acid sequence (i) recognizes the transposon, (ii) has at least 70% identity to full-length SEQ ID NO:
- 1, and (iii) comprises an amino acid substitution of D189A, V377T, E469K, K573E, E578L, or any combination thereof, when numbered in accordance with SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.