Culture media for multipotent stem cells

A growth medium for culturing mesenchymal stem cells comprising:

• a) a serum from about 1 percent to about 10 percent by volume;
• b) a fibroblast growth factor from about 1 g/mL to about 10 ng/mL;
• and
• c) either:
• i) an L-cysteine from about 0.5 mM to about 5 mM;
• or
• ii) a glutathione from about 0.5 mM to about 5 mM;
• d) an epidermal growth factor from about 1 ng/ml to about 10 ng/ml;
• e) hydrocortisone from about 10 ng/mL to about 100 ng/mL;
• f) calcium chloride from about 0.01 mM to about 0.1 mM;
• g) insulin from about 0.5 mg/mL to about 5 mg/mL;
• h) pituitary extract from about 10 μg/mL to about 100 μg/mL;
• i) selenium from about 0.1 μg/mL to about 1 μg/mL;
• j) stromal-derived factor from about 1 ng/mL to about 10 ng/mL;
• k) sodium pyruvate from about 2 mg/mL to about 20 mg/mL;
• l) transferrin from about 0.1 mg/mL to about 1 mg/mL;
• and
• m) serum free medium balanced to 100 percent volume;
• and
• wherein, a cultured mesenchymal stem cell is morphologically identical to a mesenchymal stem cell of a previous generation.