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US 11,111,479 B2Granted
Methods and kits for guided stem cell differentiation
OwnerShengyuan Yanginventor
Life of this patent
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Abstract
The present disclosure provides methods, and related kits, for directing cell attachment and spreading on a substrate and inducing isotropic spreading of cells; provides methods, and related kits, for cell sorting; and further provides methods, and related kits, for guided induction of stem cell differentiation.
Claims3
A method to modulate the relative osteogenic gene expression of a mesenchymal stem cell in the presence of osteocyte differentiation induction media by culturing the mesenchymal stem cell on a ball having a diameter and choosing a diameter between 500 μm and 6 mm, excluding 3 mm, comprising:
- culturing the mesenchymal stem cell on a ball, comprising the chosen diameter between about 500 μm and about 6 mm excluding 3 mm, wherein the level of the relative osteogenic gene expression of the mesenchymal stem cell decreases as the diameter of the substrate ball increases from about 500 μm to about 3 mm, the level of the relative osteogenic gene expression of the mesenchymal stem cell increases as the diameter of the ball increases from about 3 mm to about 6 mm and the level of the relative osteogenic gene expression of the mesenchymal stem cell on a ball of a diameter about 3 mm is comparable to that of the mesenchymal stem cell on a flat plate.
Dependencies are inferred from the claim text.