US 11,111,472 B2Granted

Delivery of biomolecules to immune cells

AssigneesMassachusetts Institute of Technology, President and Fellows of Harvard College

InventorsArmon R. Sharei, Shirley Mao, George Hartoularos, Sophia Liu, Megan Heimann, Pamela Basto, Gregory Szeto, Siddharth Jhunjhunwala, Darrell Irvine, Robert S. Langer, Klavs F. Jensen, Ulrich H. Von Andrian

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Abstract

A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.

Claims145

Dependent claims

A method for engineering of immune cell function in an immune cell comprising intracellular delivery of a compound by passing a suspension comprising said immune cell through a microfluidic device comprising a constriction and contacting the immune cell with said compound to deliver said compound into the cytosol of said immune cell, wherein the constriction has a diameter of 2 μm to 10 μm, and wherein said compound:

a) comprises a differentiation factor, a self-amplifying RNA that encodes for continual production of an encoded protein, a chimeric antigen receptor, a nucleic acid encoding a chimeric antigen receptor, a tolerogenic factor, an adjuvant, a virus, a virus-like particle, a nucleic acid that is a plasmid, a nucleic acid that is a transposon, or a nucleic acid that encodes an MHC complex;
or
b) confers the expression of a homing phenotype to the immune cell;
or
c) induces the differentiation of the immune cell into a cell with a tolerogenic phenotype;
or
d) enhances immune cell function, wherein the immune cell is a T cell, and wherein the compound that enhances T cell function is an immune checkpoint pathway inhibitor.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/523,142Filed 30 Oct 2015 · us provisional application
Priority: 31 Oct 2014
Published: US 2018/0142198 A124 May 2018
Granted: 7 Sep 2021
Term ends: Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12M 35/04
Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for…:…
A61K 39/00
Medicinal preparations containing antigens or antibodies
A61K 45/06
Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00: mixtures of active ingredients without…
A61K 49/0017
Luminescence: fluorescence in vivo

Family

Application62/073,548Us provisional application · filed 31 Oct 2014
US 2018/0142198 A1Pre-grant publication · published 24 May 2018
US 11,111,472 B2This patent

From the US filing history. Foreign family members aren’t listed.

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