Packed-bed bioreactor systems and methods of using the same
AssigneeCorning Incorporated
InventorsAnn MeeJin Ferrie, Vasiliy Nikolaevich Goral
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Abstract
A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.
Claims30
A packed-bed bioreactor system for culturing cells, the system comprising:
- a cell culture vessel comprising at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir;
- and
- a cell culture matrix disposed in the reservoir, the cell culture matrix comprising a structurally defined multi-layered substrate configured for adhering cells thereto,
- wherein a plurality of layers in the structurally defined multi-layered substrate are not separated by a spacer material or a physical barrier, and
- wherein each layer of the multi-layered substrate comprises a physical structure and a porosity that are in an ordered array that is substantially regular and uniform.
Dependencies are inferred from the claim text.