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US 11,111,310 B2Granted
Chimeric polypeptides and methods of altering the membrane localization of the same
AssigneeCell Design Labs, Inc.
InventorsStephen Santoro, Scott Coyle, Levi Rupp, Peter Emtage
Life of this patent
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Abstract
Methods and compositions are provided for reversibly localizing proteins to the exterior part of the cell surface. Compositions provided herein can include nucleic acids that encode polypeptides of interest and the ligand binding domain (LBD) of a nuclear hormone receptor. Medical applications are provided, including controlling the toxicity of CAR T cells.
Claims32
A single-chain chimeric polypeptide, wherein the single-chain chimeric polypeptide comprises:
- a transmembrane domain;
- and
- a hormone receptor ligand binding domain,
- wherein:
- the transmembrane domain and the hormone receptor ligand binding domain directly abut each other or are separated by 1 to 500 amino acids;
- the transmembrane domain and the hormone receptor ligand binding domain are not both present in the same endogenous single-chain polypeptide in a mammal;
- and
- wherein the hormone receptor ligand binding domain is a ligand binding domain of a steroid receptor.
5 more elements
A single-chain chimeric polypeptide, wherein the single-chain chimeric polypeptide comprises:
- an extracellular hormone receptor ligand binding domain;
- and
- a transmembrane domain,
- wherein:
- the extracellular hormone receptor ligand binding domain and the transmembrane domain directly abut each other or are separated by 1 to 700 amino acids;
- the transmembrane domain and the extracellular hormone receptor ligand binding domain are not both present in a single endogenous single-chain polypeptide in a mammal;
- and
- wherein the extracellular hormone receptor ligand binding domain is a ligand binding domain of a steroid receptor.
5 more elements
Dependencies are inferred from the claim text.