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US 11,111,269 B2Granted
Hydrophobic interaction protein chromatography under no-salt conditions
AssigneeBiogen MA Inc.
InventorsSanchayita Ghose, Yinying Tao
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Abstract
Various aspects and embodiments of the present disclosure relate to the purification antibodies by hydrophobic interaction chromatography under no-salt conditions.
Claims12
A method of purifying an antibody, the method comprising:
- subjecting an antibody in solution to hydrophobic interaction chromatography in flow through mode using a matrix containing hydrophobic ligands with a mobile phase buffer, wherein the mobile phase buffer does not contain a kosmotropic salt and has a pH of about 5.0 to about 7.0.
A method of purifying an antibody, the method comprising:
- providing an antibody in a solution;
- loading the solution onto a matrix containing hydrophobic ligands;
- adding a mobile phase buffer that has a pH of about 5.0 to about 7.0, wherein the mobile phase buffer does not contain a kosmotropic salt;
- and
- collecting a flow through fraction that contains the antibody.
2 more elements
Dependencies are inferred from the claim text.