Use of quaternary and tertiary ammonium cations to denature proteins
AssigneeAgilent Technologies, Inc.
InventorsMichael J. Kimzey, Francis T. Haxo, Vaishali Sharma
Life of this patent
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Abstract
Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.
Claims50
A method of denaturing a glycoprotein or glycopeptide of interest in vitro, said method comprising incubating said glycoprotein or glycopeptide in vitro with a solution comprising an effective amount of an ammonium cation sulfate or sulfonate detergent comprising (a) an aliphatic chain of 8-24 carbons, (b) a sulfate or sulfonate anion and (c) a tertiary or quaternary ammonium cation, wherein said aliphatic chain is covalently attached to said anion, for a time T to denature said glycoprotein or glycopeptide, thereby denaturing said glycoprotein or glycopeptide.
A method of denaturing a glycoprotein or glycopeptide of interest in vitro, said method comprising incubating said glycoprotein or glycopeptide in vitro with a solution comprising effective amounts of
- (a) a compound of the formula:
- R—Y−.M+, in which:
- R is a saturated or unsaturated straight chain aliphatic group with 8-13 carbon atoms;
- Y− is a sulfate or sulfonate anion;
- M+ is a cation of an element in periodic table group 1;
- and,
- the dot indicates that the cation is ionically, not covalently, associated with the Y− anion, and either
- (b) an ammonium cation detergent comprising:
- (i) an aliphatic chain of 8-24 carbons, (ii) a carboxylate anion covalently attached to said aliphatic chain and, (iii) a tertiary or quaternary ammonium cation ionically, not covalently, associated with the carboxylate anion, or
- (b′) a molar excess of quaternary ammonium cations, or of tertiary ammonium cations, or of both.
Dependencies are inferred from the claim text.