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US 11,110,683 B2Granted
Substrates comprising nano-patterning surfaces and methods of preparing thereof
AssigneeIllumina, Inc.
InventorsM. Shane Bowen, Bala Murali Venkatesan, Steven M. Barnard
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Abstract
Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
Claims17
A process of preparing a substrate, comprising:
- contacting a template having a plurality of micro-scale or nano-scale patterns with a surface of a substrate comprising a photocurable polymer layer disposed thereon;
- applying pressure to the template or substrate to transfer the plurality of micro-scale or nano-scale patterns to the photocurable polymer layer, wherein the contacting and applying are performed at room temperature;
- irradiating the photocurable polymer with UV light to cure the photocurable polymer to form a UV cured polymer layer;
- and
- separating the template from substrate;
- wherein the substrate comprises a functionalizable layer in contact with the photocurable polymer layer, wherein the functionalizable layer comprises a functionalizable hydrogel or a functionalizable polymer, and wherein the functionalizable hydrogel or functionalizable polymer comprises one or more functional groups capable of attaching to biomolecules;
- and
- wherein the one or more functional groups of the functionalizable hydrogel or the functionalizable polymer are selected from the group consisting of optionally substituted alkene, azide, optionally substituted amine, carboxylic acid, optionally substituted hydrazone, hydroxy, optionally substituted tetrazole, optionally substituted tetrazine, thiol, and combinations thereof.
5 more elements
A process of preparing a substrate, comprising:
- depositing a functionalizable layer comprising one or more functional groups onto a surface of a template comprising a plurality of micro-scale or nano-scale patterns, such that the functionalizable layer is contained inside at least a portion of said micro-scale or nano-scale patterns;
- contacting the surface of the template comprising the plurality of micro-scale or nano-scale patterns with a surface of a substrate comprising a photocurable polymer layer disposed thereon;
- applying pressure to the template or substrate to transfer said micro-scale or nano-scale patterns to the photocurable polymer layer, wherein the contacting and applying are performed at room temperature;
- irradiating the photocurable polymer with UV light to form a UV cured polymer layer;
- and;
- and
- separating the template from the substrate;
- wherein at least a portion of the functionalizable layer is transferred onto the UV cured polymer layer, wherein the functionalizable layer comprises a functionalizable hydrogel or a functionalizable polymer, and wherein the functionalizable hydrogel or functionalizable polymer comprises one or more functional groups capable of attaching to biomolecules;
- and
- wherein the one or more functional groups of the functionalizable hydrogel or the functionalizable polymer are selected from the group consisting of optionally substituted alkene, azide, optionally substituted amine, carboxylic acid, optionally substituted hydrazone, hydroxy, optionally substituted tetrazole, optionally substituted tetrazine, thiol, and combinations thereof.
7 more elements
Dependencies are inferred from the claim text.