Method for enhancing filtration yields in tangential flow filtration system

A process for enhanced recovery of a biological fluid from an activated crossflow filtration system, the process comprising:

• a. inducing an internalized pressurized countercurrent flow throughout the activated crossflow filtration system by activating an internal pump fluidically coupled to a sterile air source at a first end of the activated crossflow filtration system to cause said biological fluid to flow in a flow direction from the sterile air source toward a drain valve and opposite to a crossflow direction,
• b. recovering said biological fluid at the drain valve fluidically coupled at a second end opposite the first end of the activated crossflow filtration system,
• wherein the crossflow direction is along a fluid loop from a biofluid source container to a crossflow filtration filter and back to the biofluid source container,
• wherein the activating of the internal pump causes sterile air from the sterile air source to be introduced into the fluid loop between the biofluid source container and the crossflow filtration filter, and
• wherein said inducing of the internalized pressurized countercurrent flow comprises supplying sterile air from the sterile air source passively.