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US 11,079,584 B2Granted
Method for use in optical imaging, a system for using in optical imaging and an optical system
AssigneeCity University of Hong Kong
InventorsYajing Shen, Haojian Lu
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Abstract
A method for use in optical imaging, a system for using in optical imaging, and an optical system includes a controller arranged to determine an intermediate position of a sample upon a detection of a completion of a first movement of the sample, and to derive an optimal position associated with the intermediate position; a manipulator arranged to move the sample from the intermediate position to the optimal position with a second movement; wherein the sample is arranged to be observed using an optical instrument in the optimal position.
Claims26
A method for use in optical imaging, comprising steps of:
- determining a rough adjustment required to maintain a sample held by a rotatory positioner at approximately a center of the rotary positioner where the sample is arranged to be observed repeatedly using an optical instrument when the rotatory positioner rotates the sample at multiple angles, wherein the sample is arranged to be observed using the optical instrument in an optimal position, and the optimal position is within both a depth of field and a field of view of the optical instrument;
- and
- repeating steps of a dynamic regulation process for obtaining a plurality of optical images of the sample from different angles, the dynamic regulation process including:
- controlling the rotatory positioner by adopting the required rough adjustment to rotate the sample with a first movement to a predetermined angle, wherein the first movement includes a rotational movement about a rotation axis;
- capturing a first image of the sample upon a completion of the first movement of the sample;
- determining, based on image processing of the first image, an intermediate position of the sample upon a completion of the first movement of the sample about the rotation axis, the rotation axis being orthogonal to a direction from which the sample is arranged to be observed using the optical instrument, the sample having a three dimensional position including an X-direction, a Y-direction, and a Z-direction;
- determining, based on image processing of the first image, an offset distance between the intermediate position and the optimal position along the X-direction, the Y-direction, and the Z-direction of the three dimensional position of the sample, wherein the offset distance is caused by rotating the sample based on the rough adjustment;
- moving the rotatory positioner thereby moving the sample from the intermediate position to the optimal position with a second movement to reduce the offset distance, wherein the second movement includes a translational movement in at least two axial directions, and wherein the second movement from the intermediate position to the optimal position is arranged to provide two degrees of freedom;
- and
- capturing and storing a second image as one of the plurality of optical images of the sample being observed from the predetermined angle.
7 more elements
An optical imaging system, comprising:
- an optical instrument arranged to observe a sample positioned in an optimal position, wherein the optimal position is within both a depth of field and a field of view of the optical instrument;
- a sample holding stage having a rotatory positioner arranged to hold the sample, wherein the sample is arranged to be observed repeatedly using the optical instrument when the rotatory positioner rotates the sample at multiple angles;
- and
- a controller arranged to determine a rough adjustment required to maintain the sample at approximately a center of the rotary positioner, where the controller is further arranged to control the optical instrument and the rotatory positioner based on a dynamic regulation process so as to obtain a plurality of optical images of the sample from different angles by performing a dynamic regulation process, where the dynamic regulation process includes:
- controlling the rotatory positioner by adopting the required rough adjustment to rotate the sample with a first movement to a predetermined angle, wherein the first movement includes a rotational movement about a rotation axis;
- capturing a first image of the sample upon a completion of the first movement of the sample;
- determining, based on image processing of the first image, an intermediate position of the sample upon a completion of the first movement of the sample about the rotation axis, the rotation axis being orthogonal to a direction from which the sample is arranged to be observed using the optical instrument, the sample having a three dimensional position including an X-direction, a Y-direction, and a Z-direction;
- determining, based on image processing of the first image, an offset distance between the intermediate position and the optimal position along the X-direction, the Y-direction, and the Z-direction of the three dimensional position of the sample, wherein the offset distance is caused by rotating the sample based on the rough adjustment;
- moving the rotatory positioner thereby moving the sample from the intermediate position to the optimal position with a second movement to reduce the offset distance, wherein the second movement includes a translational movement in at least two axial directions, and wherein the second movement from the intermediate position to the optimal position is arranged to provide two degrees of freedom;
- and
- capturing and storing a second image as one of the plurality of optical images of the sample being observed from the predetermined angle.
8 more elements
Dependencies are inferred from the claim text.