Back to results
US 11,079,372 B2Granted
Polymers and conjugates comprising the same
AssigneeVentana Medical Systems, Inc.
InventorsLei Tang, Wenjun Zhang
Life of this patent
Estimating the term…
Abstract
Polymers and conjugates comprising polymers are disclosed herein. In some embodiments, the conjugates disclosed are suitable for use as detection probes in immunohistochemical assays, including multiplex immunohistochemical assays.
Claims27
A polymer of Formula (V),
- wherein
- ‘Olig’ is a single-stranded oligonucleotide sequence having between 1 and about 50 nucleotides, wherein the oligonucleotide sequence has a Tm of less than 70° C.;
- ‘Spacer’ is a saturated straight chain aliphatic group having between 4 and 32 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Linker’ is a saturated straight chain aliphatic group having between 4 and 18 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Label’ is selected from the group consisting of haptens, chromogens, and enzymes;
- R is H, a hydroxyl group, an amino group, a carbonyl group, a phosphate group, a phosphodiester group, or a cation;
- T is a group having a terminal reactive moiety;
- x is 1 or 2;
- y is 1, or 2;
- z is an integer ranging from 2 to 18;
- m is 1, or 2;
- n is 1 or 2;
- a is an integer ranging from 1 to 8;
- b is 1 or 2;
- and
- wherein any of the ‘Olig’, ‘Spacer,’ ‘Linker’ or ‘Label’ may be bonded directly to each other or through an optional group;
- and
- wherein at least one of the ‘Spacer’ and the ‘Linker’ comprises a moiety having Formula (IX):
- where d and e are each independently an integer ranging from 1 to 32,
- wherein the ‘Label’ is selected from the group consisting of a di-nitrophenyl, biotin, digoxigenin, fluorescein, a fluorescein derivative, a rhodamine, an oxazole, a pyrazole, a thiazole, a nitroaryl, a benzofuran, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, 5-nitro-3-pyrazole carbamide, 2-(3,4-dimethoxyphenyl)quinoline-4-carboxylic acid), 3-hydroxy-2-quinoxalinecarbamide, 2,1,3-benzoxadiazole-5-carbamide, and 2-acetamido-4-methyl-5-thiazolesuifonamide.
17 more elements
A polymer of Formula (V),
- wherein
- ‘Olig’ is an oligonucleotide sequence having between 1 and about 50 nucleotides, wherein the oligonucleotide sequence has a Tm of less than 70° C.;
- ‘Spacer’ is a saturated straight chain aliphatic group having between 4 and 32 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Linker’ is a saturated straight chain aliphatic group having between 4 and 18 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Label’ is selected from the group consisting of haptens, fluorophores, chromogens, enzymes and quantum dots;
- R is H, a hydroxyl group, an amino group, a carbonyl group, a phosphate group, a phosphodiester group, or a cation
- T is a group having a terminal reactive moiety;
- x is 1 or 2;
- y is 1, or 2;
- z is an integer ranging from 2 to 18;
- m is 1, or 2;
- n is 1 or 2;
- a is an integer ranging from 1 to 8;
- bis 1 or 2;
- and
- wherein any of the ‘Olig’, ‘Spacer,’ ‘Linker’ or ‘Label’ may be bonded directly to each other or through an optional group, and wherein ‘Spacer’ and ‘Linker’ are different;
- and
- wherein at least one of the ‘Spacer’ or the ‘Linker’ comprises a moiety having Formula (IX):
- where each d is independently an integer ranging from 1 to 32, and where e is an integer ranging from 2 to 32;
- and where the haptens are selected from the group consisting of pyrazoles, nitrophenyl compounds, benzofurazans, triterpenes, ureas, thioureas, rotenone, rotenone derivatives, oxazole s, thiazoles, coumarins, coumarin derivatives, nitroaryls, triterpenes, and cyclolignans.
17 more elements
A polymer of Formula (V),
- wherein
- ‘Olig’ is a single-stranded oligonucleotide sequence having between 1 and about 50 nucleotides, wherein the oligonucleotide sequence has a Tm of less than 70° C.;
- ‘Spacer’ is a saturated straight chain aliphatic group having between 4 and 32 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Linker’ is a saturated straight chain aliphatic group having between 4 and 18 carbon atoms, wherein the aliphatic group is unbranched and unsubstituted, and optionally includes one or more oxygen atoms;
- ‘Label’ is a fluorophore or a hapten;
- R is H, a hydroxyl group, an amino group, a carbonyl group, a phosphate group, a phosphodiester group, or a cation;
- T is a group having a terminal reactive moiety;
- x is 1 or 2;
- y is 1, or 2;
- z is an integer ranging from 3 to 6;
- m is 1, or 2;
- n is 1 or 2;
- a is an integer ranging from 1 to 8;
- bis 1 or 2;
- and
- wherein any of the ‘Olig’, ‘Spacer,’ ‘Linker’ or ‘Label’ may be bonded directly to each other or through an optional group;
- and
- wherein at least one of the ‘Spacer’ and the ‘Linker’ comprises a moiety having Formula (IX):
- where d and e are each independently an integer ranging from 1 to 32.
16 more elements
Dependencies are inferred from the claim text.