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US 11,078,543 B2Granted
Detecting pancreatic high-grade dysplasia
AssigneeMayo Foundation for Medical Education and Research
InventorsDavid A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
Life of this patent
Estimating the term…
Abstract
Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).
Claims7
A method for characterizing a biological sample comprising:
- measuring a methylation level of a CpG site for twenty-two or fewer genes in a biological sample of a human through
- treating genomic DNA in the biological sample with bisulfite;
- amplifying the bisulfite-treated genomic DNA using a set of primers for each of the selected twenty-two or fewer genes;
- and
- determining the methylation level of the CpG site by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, or bisulfite genomic sequencing PCR,
- wherein the twenty-two or fewer genes are selected from BMP3, NDRG4, ABCB1, AK055957, C13ORF18, CD1D, CLEC11A, DLX4, ELMO1, EMX1, FER1L4, FRMD4A, GRIN2D, HOXA1, LRRC4, PRKCB, SP9, ST6GAL2, ST8SIA1, TBX15, VWC2, and ZNF781;
- wherein the twenty-two or fewer genes at least include AK055957 and ST8SIA1;
- wherein the twenty-two or fewer genes at least include one of the following:
- DLX4, EMX1, FRMD4A, LRRC4, SP9, TBX15, and ZNF781.
6 more elements
A method for characterizing a biological sample comprising:
- measuring a methylation level of a CpG site for three or more genes in a biological sample of a human through
- treating genomic DNA in the biological sample with bisulfite;
- amplifying the bisulfite-treated genomic DNA using a set of primers for each of the selected three or more genes;
- and
- determining the methylation level of the CpG site by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, or bisulfite genomic sequencing PCR,
- wherein the three or more genes are selected from BMP3, NDRG4, ABCB1, AK055957, C13ORF18, CD1D, CLEC11A, DLX4, ELMO1, EMX1, FER1L4, FRMD4A, GRIN2D, HOXA1, LRRC4, PRKCB, SP9, ST6GAL2, ST8SIA1, TBX15, VWC2, and ZNF781;
- wherein the three or more genes at least include AK055957 and ST8SIA1;
- wherein the three or more genes at least include one of the following:
- DLX4, EMX1, FRMD4A, LRRC4, SP9, TBX15, and ZNF781.
6 more elements
Dependencies are inferred from the claim text.