Synthetic nucleic acid spike-ins

A composition comprising at least 1,000 unique synthetic nucleic acid sequences wherein each of said at least 1,000 unique synthetic nucleic acids comprise sequences identified in SEQ ID NO:

• 119 or SEQ ID NO:
• 120.

A composition comprising:

• (a) a biological sample from a tissue or body fluid;
• and
• (b) at least 1,000 synthetic nucleic acids comprising nucleic acid sequences that are not naturally occurring wherein at least 1,000 of said at least 1,000 synthetic nucleic acids comprising nucleic acid sequences that are not naturally occurring comprise one or more identifying tag regions positioned downstream or upstream of a unique variable region, wherein said unique variable region comprises at least five degenerate bases, and wherein said at least 1,000 of said at least 1,000 synthetic nucleic acids comprising nucleic acid sequences that are not naturally occurring have sequences that are unique to each other.

A composition comprising:

• (a) genomic nucleic acids that are not attached to an adapter;
• and
• (b) at least 1,000 synthetic nucleic acids comprising nucleic acid sequences that are not naturally occurring wherein at least 1,000 of said at least 1,000 synthetic nucleic acids comprising nucleic acid sequences that are not naturally occurring comprise one or more identifying tag regions positioned downstream or upstream of a unique variable region, wherein said unique variable region comprises at least five degenerate bases, and wherein said at least 1,000 of said at least 1,000 synthetic nucleic acid sequences have sequences that are unique to each other.

A composition comprising at least 1,000 synthetic spike-in nucleic acids comprising non-natural nucleic acid sequences that are not naturally occurring wherein at least 1,000 of said at least 1,000 unique synthetic nucleic acids comprising non-natural nucleic acid sequences that are not naturally occurring comprises one or more identifying tag regions positioned downstream or upstream of a unique variable region, wherein said unique variable region comprises at least five degenerate bases and wherein said degenerate bases are split into at least two groups separated by at least four contiguous nucleotides wherein each of said non-natural nucleic acid sequences that are not naturally occurring is unique to each other.