Method for quantifying and/or detecting human male DNA

A method for detecting, assessing the status of and/or quantifying the fraction of male DNA in a sample, wherein the method comprises amplification of a multicopy locus within the Y-chromosome (MCL-Y), and wherein the amplification is performed using at least one primer selected from the group consisting of:

• a. SEQ ID NO. 1;
• b. SEQ ID NO. 2;
• c. the reverse complement of SEQ ID NO. 1;
• d. the reverse complement of SEQ ID NO. 2;
• e. a primer that shares at least 90% sequence identity with SEQ ID NO. 1;
• f. a primer that shares at least 90% sequence identity with SEQ ID NO. 2;
• g. the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 1;
• and
• h. the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2.