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US 11,078,520 B2Granted
Method for generating a three-dimensional nucleic acid containing matrix
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
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Abstract
Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
Claims31
A method for analysis, comprising:
- (a) providing a biological sample comprising a plurality of cells, wherein a cell of said plurality of cells comprises a plurality of cellular nucleic acids;
- (b) generating a three-dimensional matrix comprising cellular nucleic acids of said plurality of cellular nucleic acids or derivatives of said cellular nucleic acids, wherein said cellular nucleic acids or derivatives of said cellular nucleic acids have a three-dimensional position in said three-dimensional matrix;
- and
- (c) detecting signals from said cellular nucleic acids or derivatives of said cellular nucleic acids to identify said three-dimensional position in said three-dimensional matrix.
1 more element
A method for analysis, comprising:
- (a) providing a biological sample comprising a plurality of cells, wherein a cell of said plurality of cells comprises a plurality of cellular nucleic acids;
- (b) generating a plurality of amplicons from said plurality of cellular nucleic acids;
- (c) generating a three-dimensional matrix comprising amplicons of said plurality of amplicons, wherein said amplicons are covalently attached to said three-dimensional matrix, and wherein said amplicons have a three-dimensional position in said three-dimensional matrix;
- and
- (d) hybridizing fluorescently labeled oligonucleotides to said amplicons and imaging said amplicons to identify said three-dimensional position in said three-dimensional matrix.
2 more elements
Dependencies are inferred from the claim text.