Hemoglobin A1c measurement method and measurement kit

A reagent kit for measurement of hemoglobin A1c in a sample comprising ingredients (1) and (2) below:

• (1) a modified amadoriase that acts directly on hemoglobin A1c and generates hydrogen peroxide;
• and
• (2) a reagent for measurement of hydrogen peroxide,
• wherein the modified amadoriase is selected from:
• (i) a modified amadoriase comprising the amino acid sequence of SEQ ID NO:
• 1, and wherein the amino acid at position 62 is substituted with aspartic acid;
• the amino acid at position 63 is substituted with histidine;
• the amino acid at position 102 is substituted with lysine;
• the amino acid at position 106 is substituted with lysine;
• the amino acid at position 110 is substituted with leucine;
• the amino acid at position 113 is substituted with lysine;
• and the amino acid at position 355 is substituted with serine, and
• (ii) a modified amadoriase comprising an amino acid sequence that is at least 90% identical to the amadoriase of (i).