Methods for identifying targets for antimicrobial and antiproliferative compounds and compositions therefrom

A method of identifying one or more antimicrobial molecular targets in a budding yeast cell comprising:

• growing the budding yeast cell in vitro under infection mimetic conditions to produce a metabolically synchronous population of budding yeast cells;
• sampling continuous cultures of the metabolically synchronous population of budding yeast cells over time;
• and
• identifying genes that are differentially regulated in the metabolically synchronous population of the budding yeast cells when grown in vitro under the infection mimetic conditions as compared to the budding yeast cells grown in vitro under standard laboratory conditions, wherein the infection mimetic conditions are of a site of an infection by the budding yeast cell, and the site of the infection is in an animal.