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US 11,078,512 B2Granted
Compositions for cell bioproduction and methods of using the same
AssigneeBristol-Myers Squibb Company
InventorsJun Tian, Jianlin Xu, Qin He
Life of this patent
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Abstract
The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.
Claims5
A cell culture process for the production of protein, comprising:
- a) culturing CHO cells in a basal media which produce a protein of interest in cell culture under conditions that allow for protein production;
- and
- b) feeding the cell culture with a feed media, wherein 1 mM rosmarinic acid is added to the feed media,
- wherein viable cell density is increased compared to the same cell culture without supplementation of rosmarinic acid.
1 more element
Dependencies are inferred from the claim text.