Methods of fermenting mixtures that include di- and tri-saccharides formed at low temperature using a maltophilic yeast

A method for producing ethanol from a plant matter, comprising the steps of:

• liquefying of grain solids by treating a combination of the grain solids and water with an alpha-amylase at a concentration between 0.5 to 4 wt. % of the grain solids at a temperature of 70° C. or lower to form a grain slurry;
• adding a maltogenic alpha-amylase;
• a maltophilic strain of Saccharomyces;
• and beta amylase to the grain slurry to form a mixture;
• and
• fermenting the mixture at a temperature of about 38° C. or lower,
• wherein the maltophilic strain of Saccharomyces is a GRR1 knock-out of Ethanol Red.