Vector for targeting the human Rosa26 gene
AssigneeIcahn School of Medicine at Mount Sinai
InventorsGordon Keller, Stefan Irion, Herve Luche, Paul Gadue, Hans J. Fehling
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Abstract
The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.
Claims11
A targeting vector comprising an expression cassette comprising a nucleic acid encoding a protein, wherein said nucleic acid is heterologous to a human Rosa 26 gene, said expression cassette flanked by DNA sequences homologous to the human Rosa26 gene.
Dependencies are inferred from the claim text.