A method of increasing nitrogen use efficiency, yield, biomass, growth rate, and/or tolerance to nitrogen deficiency of a plant, wherein the nitrogen deficiency is a growth condition including an applied level of nitrogen which is below a level needed for a metabolism, growth, reproduction and/or viability of a control plant of the same species, the method comprising:
- (a) expressing within the plant a heterologous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence as set forth by SEQ ID NO:
- 475, or a homologous polypeptide having at least 83% sequence identity and conservative amino acid substitution(s) with respect to the amino acid sequence set forth by SEQ ID NO:
- 475, and
- (b) selecting said plant expressing said heterologous polynucleotide for an increased nitrogen use efficiency, yield, biomass, growth rate, and/or tolerance to nitrogen deficiency as compared to a control plant of the same species which is grown under the same growth conditions,
- thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, and/or the tolerance to nitrogen deficiency of the plant.