Optimized vector for delivery in microbial populations
AssigneesEligo Bioscience, Institut Pasteur
InventorsAntoine Decrulle, Jesus Fernandez Rodriguez, Xavier Duportet, David Bikard
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Abstract
The present invention relates to a vector, preferably included in a delivery vehicle, comprising no more than 100, preferably no more than 10, restriction sites recognized by the restriction enzymes encoded by each bacterium of a group of bacteria of interest. The invention also relates to the use of said vector, preferably included in a delivery vehicle, as a drug, especially in the treatment of a disease in a patient in need thereof.
Claims20
An engineered bacteriophage or packaged phagemid, for DNA delivery into Klebsiella pneumoniae strains, wherein said engineered bacteriophage or packaged phagemid has been genetically engineered to remove one or more of the restriction sites selected from the group consisting of CAGNNNNNNCGT (SEQ ID NO:
- 93);
- GAAYNNNNNNNCTGG (SEQ ID NO:
- 94);
- CGANNNNNNNNTGCC (SEQ ID NO:
- 95);
- ACGNNNNNGTTG (SEQ ID NO:
- 96) and CGCATC corresponding to restriction enzymes encoded by Klebsiella pneumoniae strains.
Dependencies are inferred from the claim text.