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US 11,078,482 B2Granted
Duplex sequencing using direct repeat molecules
AssigneeGenome Research Limited
InventorsRobert Osborne, Christopher Laumer
Life of this patent
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Abstract
Described herein is a population of direct repeat molecules, where each molecule of the population contains a direct repeat composed of sequences that are amplified from the opposite strands of a double-stranded fragment of genomic DNA. Within each molecule, the first repeat (referred to as TOP) is amplified from the one strand of a double-stranded fragment of genomic DNA and the second repeat (referred to as BOT′) is amplified from the other strand of the same fragment of double-stranded fragment of genomic DNA.
Claims21
A population of direct repeat molecules having a strand of the formula:
- X-TOP-J-BOT′-Y, wherein:
- (i) each direct repeat molecule contains one copy of TOP and one copy of BOT′ and within each repeat molecule TOP and BOT′ are amplified from opposite strands of a double-stranded fragment of genomic DNA and are identical except for positions that correspond to damaged nucleotides in the double-stranded fragment of genomic DNA or errors that occur during amplification;
- (ii) TOP and BOT′ vary in the population and have a median length of at least 50 nucleotides;
- (iii) X and Y are different, non-complementary sequences of at least 10 nucleotides in length that do not vary in the population;
- and
- (iv) J is a junction sequence.
3 more elements
Dependencies are inferred from the claim text.