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US 11,078,479 B2Granted
Polypeptide display libraries and methods of making and using thereof
AssigneeThe Regents of the University of California
InventorsPatrick S. Daugherty, Paul H. Bessette, Jeffrey Rice
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Abstract
Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.
Claims18
A nucleic acid encoding a fusion protein, the fusion protein comprising:
- an outer membrane protein and a passenger polypeptide,
- wherein the outer membrane protein is a circularly permuted variant of a native outer membrane protein,
- wherein a native N-terminus and a native C-terminus of the native outer membrane protein are joined by a linker,
- wherein the outer membrane protein comprises a non-native N-terminus and a non-native C-terminus derived from an extracellular loop of the native outer membrane protein,
- wherein the passenger polypeptide is fused to the non-native N-terminus or the non-native C-terminus of the outer membrane protein, and
- wherein the native outer membrane protein is a bacterial outer membrane protein is OmpA or OmpX.
3 more elements
Dependencies are inferred from the claim text.