Methods of capturing cell-free methylated DNA and uses of same
AssigneesSinai Health System, University Health Network
InventorsDaniel Diniz De Carvalho, Shu Yi Shen, Rajat Singhania
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Abstract
There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
Claims23
A method of capturing cell-free methylated DNA from a sample having less than 100 ng of cell-free DNA, comprising the steps of:
- a. subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA;
- b. adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated;
- c. denaturing the sample;
- and
- d. capturing cell-free methylated DNA using a binder selective for methylated polynucleotides;
- wherein the cell-free DNA from the sample and the first amount of filler DNA together comprises at least 50 ng of total DNA.
Dependencies are inferred from the claim text.