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US 11,078,467 B2Granted
Highly active self-sufficient nitration biocatalysts
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYousong Ding, Ran Zuo
Life of this patent
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Abstract
The disclosure relates to the field of fusion proteins. In some aspects, the disclosure relates to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosure relates to corn-pounds produced by artificial cytochrome P450 enzymes.
Claims10
A fusion protein comprising:
- (i) a TxtE enzyme comprising the amino acid sequence set forth in SEQ ID NO:
- 56 or 58;
- (ii) an amino acid linker that is between 14 and 16 amino acids in length;
- and,
- (iii) a catalytic domain of a CYP102A1 (P450BM3) reductase enzyme;
- wherein the linker joins (iii) to a terminus of (i);
- wherein the fusion protein comprises increased nitration activity compare to cytochrome p450 enzyme.
4 more elements
Dependencies are inferred from the claim text.