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US 11,078,455 B2Granted
Closed culture vessel for adherent cells
OwnerSeiichi Yokooinventor
Life of this patent
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Abstract
The present invention provides a culture vessel with which cells can be aseptically cultured and aseptically transferred to a place where the cells are to be used (such as an operating room) and from which the cells can be simply taken out. The present invention provides a closed culture vessel including an openable airtight vessel, and a cell culture member disposed in the airtight vessel and including a cell culture surface, in which the cell culture member is provided detachably from the airtight vessel.
Claims7
A closed cell culture system, comprising:
- (i) a closed culture vessel for adherent cells comprising:
- a gas permeable cell culture bag;
- and
- an amnion membrane;
- (ii) medium supply means for supplying a culture medium to the gas permeable cell culture bag;
- and
- (iii) medium discharge means for discharging the culture medium from the gas permeable cell culture bag,
- wherein the amnion membrane is provided inside the gas permeable cell culture bag;
- wherein the amnion membrane is provided detachably from the gas permeable cell culture bag;
- wherein the closed culture vessel comprises no culture medium;
- wherein the supply means and the discharge means are aseptically closed;
- and
- wherein the gas permeable cell culture bag has been aseptically sealed.
10 more elements
A method, comprising:
- providing a closed culture vessel for adherent cells, comprising:
- a gas permeable cell culture bag having a cell inlet;
- and an amnion membrane,
- wherein the amnion membrane is provided inside the gas permeable cell culture bag,
- wherein the amnion membrane is provided detachably from the gas permeable cell culture bag;
- wherein the closed culture vessel comprises no culture medium;
- and
- wherein the cell inlet is aseptically closed with a rubber plug,
- introducing an adhesive cell in a culture medium at least onto the amnion membrane inside the bag through the rubber plug by using a syringe equipped with a needle that can penetrate through the rubber plug to allow the adhesive cell to attach to the amnion membrane,
- sealing the cell inlet of the gas permeable cell culture bag to include a space therein formed by filling of the gas permeable cell culture bag with a culture medium and optionally a gas, thereby protecting a cell culture surface of the amnion membrane and cells adhered to the amnion membrane from physical damage, and then,
- culturing the cell on the amnion membrane to form a cell sheet.
8 more elements
Dependencies are inferred from the claim text.