Circular tandem repeat proteins
AssigneeFred Hutchinson Cancer Research Center
InventorsPhilip Bradley, Barry L. Stoddard
Life of this patent
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Abstract
Circular handed alpha-helical repeat proteins are described. The repeat proteins have a number of uses as scaffolds for geometrically precise, arrayed presentation of cell-signaling or immune-related protein and peptide epitopes, as well as numerous other therapeutic, diagnostic, and nanotechnological uses.
Claims20
A protein having the formula:
- (a-b-x-y)n wherein
- a and x represent linker sequences;
- b represents an amino acid sequence that forms an alpha (α) helix;
- y represents an amino acid sequence that forms a second α helix;
- n=3, 6, 9, 12, or 24;
- the protein is handed, and
- the N- and C-termini of the protein create a circular architecture.
A circular, handed protein comprising at least three α-helical structures wherein each α-helical structure comprises an outer α helix and an inner α helix joined by a flexible linker and wherein each α-helical structure has at least 95% sequence identity with an adjacent α-helical structure.
A protein having the formula:
- (d-a-b-x-y)n;
- (a-d-b-x-y)n;
- (a-b-d-x-y)n;
- (a-b-x-d-y)n or (a-b-x-y-d)n wherein
- a and x represent linker sequences;
- b represents an amino acid sequence that forms an alpha (α) helix;
- y represents an amino acid sequence that forms a second α helix;
- d represents a functional domain;
- n=3, 6, 9, 12, or 24;
- the protein is handed, and
- the N- and C-termini of the protein create a circular architecture.
Dependencies are inferred from the claim text.