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US 11,077,230 B2Granted
Decellularising tissue
AssigneeVideregen Limited
InventorTahera Ansari
Life of this patent
Estimating the term…
Abstract
The invention provides a method for decellularising a tissue the method comprising the steps of: a) adding a tissue to be decellularised to a decellularisation vessel; b) adding a decellularisation medium comprising at least one detergent and/or at least one enzyme to the vessel; c) introducing a supercritical fluid to the vessel; and d) maintaining contact of the tissue with the supercritical fluid for a time sufficient to substantially decellularise the tissue.
Claims14
A method for decellularising a tissue comprising the steps of:
- a) adding a tissue to be decellularised to a decellularisation vessel;
- b) adding a decellularisation medium comprising at least one detergent and at least one enzyme selected from the group consisting of a deoxyribonuclease and a ribonuclease to the vessel;
- c) introducing supercritical carbon dioxide to the vessel with the tissue to be decellularised and the decellularisation medium present;
- and
- d) maintaining contact of the tissue with the supercritical carbon dioxide for a time sufficient to substantially decellularise the tissue.
2 more elements
A method of manufacturing a tissue implant or scaffold comprising the steps of:
- a) adding a tissue to be decellularised to a decellularisation vessel;
- b) adding a decellularisation medium comprising at least one detergent and at least one enzyme selected from the group consisting of a deoxyribonuclease and a ribonuclease to the vessel;
- c) introducing supercritical carbon dioxide to the vessel with the tissue to be decellularised and the decellularisation medium present;
- and
- d) maintaining contact of the tissue with the supercritical carbon dioxide and the decellularisation medium for a time sufficient to substantially decellularise the tissue.
2 more elements
Dependencies are inferred from the claim text.